Yes.
Non-repairable microscope auto-focus module
The lab where I started my PhD had a fully automated epi-fluorescence microscope (IX81).
We relied heavily on a hardware auto-focus module (ZDC) for quantitative time course imaging of single yeast cells.
Three years ago it stopped working, and we have been unable to get someone to repair it. The product is no longer supported, and Olympus no longer provides support of any kind in our country.
This means that the quality of the time series data has a much larger dispersion, which is bad for the kind of questions we asked. For example, we can no longer reliably quantify fluorecence localization over time.
For the record, yeasts are tiny, and consistent focus is very important. Hundreds of images are acquired during each experiment, so automated focusing is important.
Ice on the camera sensor
Same story, different device.
The seal in the camera broke, and no one knows how to repair it. The camera had been performing excellently for decades until that moment, when atmospheric air entered the chamber and ice crept onto the CCD sensor (preventing any decent imaging).
In theory it has a service port for resetting the conditions it requires, but the manuals mention nothing, and the company does not offer service in our country. The more knowledgeable folk around did not want to risk doing surgery on it without offering guarantees.
This set us back a year, until we were forced to spend a large part of the lab’s funds on a new camera. The old camera is now sitting on a drawer, fully functional, waiting for someone that knows how to do some maintenance.
Overall
As usual around here, the larger unavoidable cause for all of this is a general scarcity of resources, that makes everything work like #$%&/.
Then again, the companies are really secretive, and will not let out any useful information for repair (even when they will not offer to do it themselves).
So, because those problems will not be solved soon enough, from a practical perspective open source hardware is the best way to go. Had our equipment been OScH, we would have been spared of a lot of trouble.
The microscope is our main observation tool, so we are forced to lower the bar of what we can achieve, and settle for less impact (and then less funding ).