Great to see it is working!
Still happy to have seen nuclei in cheek cells with the OpenFlexure, and looking forward to not only finishing the v6 build (with better accessibility for the filter cube and reflection illumination), but seeing some comets.
Just to clarify, for future reference, the quoted list of Comar filters above only was wrong for the emission filter. In fact a more ‘long-pass’ filter, the Comar 510 IY 116 is what was needed to make this work (sybr-safe stained cells). The images from a couple of days ago were still with our old 10x Fluotar objective, for instance.
one more point - the LED was a simple blue one, just powered by the 5V pins of the Raspberry Pi!