I just chatted briefly to one of my biological collaborators about sybr-safe, and she mentioned it was way brighter under UV light. Are you sure 460-470nm is the right wavelength? The filter set described in that thread was for GFP/fluorescein, but perhaps you actually need a UV LED (and corresponding filters). You might get away with a UV LED and the existing filters, but perhaps you should remove the excitation filter, that might be attenuating the light. Have you checked the spectra of the filters and the dye? Sorry if that’s an obvious question, but sometimes obvious questions are worth asking! Posting/linking to the relevant spectra here would be helpful for anyone else reproducing what you are doing.
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