One hour Covid test protocol using OT-2, plus another open source pipetting robot

Interesting to see the OpenTrons OT-2 mentioned in this 1-hour Covid test protocol:

Bonus, a low-cost open source pipetting robot:


and one more World Wide Series | Open Neuroscience - 02/04/2021 - Anton Nikolaev and Pavel Katunin - YouTube

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I’m so glad to see so much work going on in OSH pipetting bots :partying_face:

A couple mates and I are also making one from scratch, also with It’s own GUI.

It’s certainly not ready, and documentation is a mess… but here are a couple pics :stuck_out_tongue:

We’d be glad to join efforts with others :slight_smile:

Making these things is really challenging IMO.


I’m interested! do you have more info?


These are the references i’ve collected so far:

Campbell, Robert A. A., Robert W. Eifert, and Glenn C. Turner. 2014. ‘Openstage: A Low-Cost Motorized Microscope Stage with Sub-Micron Positioning Accuracy’. Edited by Nikolas K. Haass. PLoS ONE 9 (2): e88977.

Carvalho, Matheus C., and Rachel H. Murray. 2018. ‘Osmar, the Open-Source Microsyringe Autosampler’. HardwareX 3 (April): 10–38.

Carvalho, Matheus C., Christian J. Sanders, and Ceylena Holloway. 2018. ‘Auto-HPGe, an Autosampler for Gamma-Ray Spectroscopy Using High-Purity Germanium (HPGe) Detectors and Heavy Shields’. HardwareX 4 (October): e00040.

Dettinger, Philip, Tobias Kull, Geethika Arekatla, Nouraiz Ahmed, Yang Zhang, Florin Schneiter, Arne Wehling, et al. 2021. ‘Open-Source Personal Pipetting Robots with Live-Cell Incubation and Microscopy Compatibility’. Preprint. Bioengineering.

Katunin, Pavel, Ashley Cadby, and Anton Nikolaev. 2020. ‘An Open-Source Experimental Framework for Automation of High-Throughput Cell Biology Experiments’. Preprint. Cell Biology.

‘Making a Opentrons Compatible Liquid Handling Robot’. n.d. Making a Opentrons compatible liquid handling robot – Pipette Jockey.

Vonesch, Sibylle C, Danila Bredikhin, Nikolay Dobrev, Laura Villacorta, Rozemarijn Kleinendorst, Elisabetta Cacace, Julia Flock, et al. 2020. ‘McQ – An Open-Source Multiplexed SARS-CoV-2 Quantification Platform’. Preprint. Infectious Diseases (except HIV/AIDS).


some of mine:
maybe you can add your references there too.
like this one: