GOSH Community Forum

Microscope and other recent Optics discussions

hi Marc
with @ffederici we are working with local beer brewers on a simple microscope to count and check yeast viability,
the design is a remix of previous open souce microscopes.
This last prototype is based on a C270 Logitech webcam and a 3D printed base to hold and focus an M12 lens. It has a screw focusing design manipulator was inspired on the work of Tristan-Landin et al. (2019). The illumination is the one of OpenFlexure.
GIT: https://gitlab.com/nanocastro/microbrew


saludos
nano

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Omg I love this. @nanocastro we should talk because we want to use the openflexure this winter for automated cell counts also but haven’t started cause we’re finishing other work. If you have any useful learnings so far would love to hear it.

We are running a public survey of Food and Soil quality from 25 sratesbin the US across 6 crops types to identify correlations between food nutrient density, soil quality, and farm practices. We’re also using that survey to attempt to calibrate two things 1) crop nutrient density and 2) soil total organic carbon using the 10 wavelength reflectometer.

Survey info - lab.realfoodcampaign.org
Reflectometer - https://bionutrient.org/site/bionutrient-meter/technicals, https://gitlab.com/our-sci/reflectance-spec-PCB

Also @dusjagr check out the GOAT community also (forum.goatech.org) and specifically OpenTEAM (openteam.community). If you’re meeting big wigs, that’s a large scale, ardently open source collaboration that’s well funded and supported. May be a another useful reference for the naysayers.

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Hi @gbathree
Regarding the automatic cell counting we did not advance so much. These are our initial thoughts about it.
For counting we are trying to avoid the Neubauer chamber with a microfluidic slide. We are now waiting for the slides to arrive.
On the ag-tech side we want to assemble a few openflexures for soil microscopy so it would be great if we have a call.
Abrazo

Recently bought these:

Very thin (as little as .7ml and .3ml), and they are pretty cheap ($5 per)… and they are reusable! They are not perfect in terms of their thickness, I’m sure there’s some variability, but maybe we can use the scope itself at a known distance to calibrate the depth of a given slide… so rather than paying for a really expensive standard thickness slide, we can use the scope or something else to calibrate each ‘slide’ each time.

I don’t know, just an idea, would love to solve this problem at a low cost, and would love to hear about your experiences so far.